Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Determination of antioxidant and anti-melanogenesis activities of Indonesian Lai, Durio kutejensis [Bombacaceae (Hassk) Becc] fruit extract

Enos Tangke Arung¹ , Wiwin Suwinarti¹, Medi Hendra², Supomo .¹, Irawan Wijaya Kusuma¹, Dwi Caesarina Nanda Puteri¹, Huseyin Avni Eroglu⁴, Yong-ung Kim⁵, Kuniyoshi Shimizu⁶, Hiroya Ishikawa⁷

¹Faculty of Forestry; ²Faculty of Mathematics and Science, Mulawarman University; ³Samarinda Academy of Pharmacy, Samarinda, 75123, Indonesia; ⁴Faculty of Veterinary Medicine, Department of Physiology, University of Kafkas, Turkey; ⁵Department of Herbal Pharmaceutical Engineering, College of Herbal Bio-industry,Daegu Haany University, Gyeongsangbuk-do, South Korea; ⁶Faculty of Agriculture, Kyushu University; ⁷International College of Arts and Sciences, Fukuoka Women's University, Fukuoka, 813-8529, Japan.

For correspondence:- Enos Arung Email: tangkearung@yahoo.com Tel:+62-541-737081

Received: 14 May 2014 Revised: 27 November 2014 Published: 30 January 2015

Citation: Arung ET, Suwinarti W, Hendra M, . S, Kusuma IW, Puteri DC, et al. Determination of antioxidant and anti-melanogenesis activities of Indonesian Lai, Durio kutejensis [Bombacaceae (Hassk) Becc] fruit extract. Trop J Pharm Res 2015; 14(1):41-46 doi: 10.4314/tjpr.v14i1.7

© 2015 The authors.
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Abstract

Purpose:To investigate the antioxidant and anti-melanogenesis activities of Durio kutejensis [Bombacaceae (Hassk.) Becc] fruit extract.

Methods:The fruit flesh of D. kutejensis was extracted successively with n-hexane, ethyl acetate/EtOAc, and ethanol/EtOH at room temperature repeatedly. The extracts were concentrated in vacuo to yield their residues. Antioxidant properties were analyzed by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS, superoxide dismutase (SOD)-like activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and oxygen radical absorbance capacity (ORAC) while anti-melanogensis activity was evaluated by tyrosinase enzyme activity and B16 melanoma cell assays (melanin inhibition and cytotoxicity).

Results:The extract (200 µg/mL) showed melanin inhibition by inhibiting melanin formation in B16 melanoma cell by 47 % without cytotoxicity but did not inhibit tyrosinase enzyme activity. The extract (1 - 1000 µg/mL) also exhibited some level of antioxidant activity including ORAC (0.04 ± 0.00 µmol TE/mg at 950 µg/mL), ABTS (1.0 ± 0.2 % at 100.8 µg/mL), SOD (IC50, 76.00 ± 14.6 µg/mL, and DPPH (21.5 ± 0.7 % at 97.39 µg/mL extract concentration).

Conclusion:The fruit extract of Durio kutejensis has antioxidant properties with a potential for treating hyperpigmentation and for use as a skin-lightening agent.

Keywords: Durio kutejensis, Antioxidant, Anti-melanogenesis, B16 Melanoma cell Hyperpigmentation, Skin-lightening agent